14-12-2025
جامعة الملك عبدالعزيز
KING ABDULAZIZ UNIVERSITY
Center of Excellence In Genomic Medicine Research
Document Details
Document Type
:
Article In Journal
Document Title
:
Investigation of the STR loci noise distributions of PowerSeq™ Auto System
Investigation of the STR loci noise distributions of PowerSeq™ Auto System
Document Language
:
English
Abstract
:
AIM: To characterize the noise and stutter distribution of 23 short tandem repeats (STRs) included in the PowerSeqTM Auto System. METHODS: Raw FASTQ files were analyzed using STRait Razor v2s to display alleles and coverage. The sequence noise was divided into several categories: noise at allele position, noise at -1 repeat position, and artifact. The average relative percentages of locus coverage for each noise, stutter, and allele were calculated from the samples used for this locus noise analysis. RESULTS: Stutter products could be routinely observed at the -2 repeat position, -1 repeat position, and +1 repeat position of alleles. Sequence noise at the allele position ranged from 10.22% to 28.81% of the total locus coverage. At the allele position, individual noise reads were relatively low. CONCLUSION: The data indicate that noise generally will be low. In addition, the PowerSeqTM Auto System could capture nine flanking region single nucleotide polymorphisms (SNPs) that would not be observed by other current kits for massively parallel sequencing (MPS) of STRs.
ISSN
:
1332-8166
Journal Name
:
Croatian medical journal
Volume
:
58
Issue Number
:
3
Publishing Year
:
1438 AH
2017 AD
Article Type
:
Article
Added Date
:
Tuesday, June 25, 2019
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
Xiangpei Zeng
Zeng, Xiangpei
Researcher
Doctorate
Xiangpei.Zeng@unthsc.edu
Jonathan L. King
King, Jonathan L.
Researcher
Doctorate
Bruce Budowle
Budowle, Bruce
Researcher
Doctorate
Files
File Name
Type
Description
44536.pdf
pdf
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