14-12-2025
جامعة الملك عبدالعزيز
KING ABDULAZIZ UNIVERSITY
Center of Excellence In Genomic Medicine Research
Document Details
Document Type
:
Article In Journal
Document Title
:
A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysis
A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysis
Document Language
:
English
Abstract
:
Amplicon (targeted) sequencing by massively parallel sequencing (PCR-MPS) is a potential method for use in forensic DNA analyses. In this application, PCR-MPS may supplement or replace other instrumental analysis methods such as capillary electrophoresis and Sanger sequencing for STR and mitochondrial DNA typing, respectively. PCR-MPS also may enable the expansion of forensic DNA analysis methods to include new marker systems such as single nucleotide polymorphisms (SNPs) and insertion/deletions (indels) that currently are assayable using various instrumental analysis methods including microarray and quantitative PCR. Acceptance of PCR-MPS as a forensic method will depend in part upon developing protocols and criteria that define the limitations of a method, including a defensible analytical threshold or method detection limit. This paper describes an approach to establish objective analytical thresholds suitable for multiplexed PCR-MPS methods. A definition is proposed for PCR-MPS method background noise, and an analytical threshold based on background noise is described.
ISSN
:
1932-6203
Journal Name
:
PLOS ONE
Volume
:
12
Issue Number
:
5
Publishing Year
:
1438 AH
2017 AD
Article Type
:
Article
Added Date
:
Wednesday, June 26, 2019
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
Brian Young
Young, Brian
Researcher
Doctorate
brian@nichevision.com
Jonathan L. King
King, Jonathan L.
Researcher
Doctorate
Bruce Budowle
Budowle, Bruce
Researcher
Doctorate
Luigi Armogida
Armogida, Luigi
Researcher
Doctorate
Files
File Name
Type
Description
44537.pdf
pdf
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