14-12-2025
جامعة الملك عبدالعزيز
KING ABDULAZIZ UNIVERSITY
Center of Excellence In Genomic Medicine Research
Document Details
Document Type
:
Article In Journal
Document Title
:
Flanking Variation Influences Rates of Stutter in Simple Repeats
Flanking Variation Influences Rates of Stutter in Simple Repeats
Document Language
:
English
Abstract
:
It has been posited that the longest uninterrupted stretch (LUS) of tandem repeats, as defined by the number of exactly matching repeating motif units, is a better predictor of rates of stutter than the parental allele length (PAL). While there are cases where this hypothesis is likely correct, such as the 9.3 allele in the TH01 locus, there can be situations where it may not apply as well. For example, the PAL may capture flanking indel variations while remaining insensitive to polymorphisms in the repeat, and these haplotypic changes may impact the stutter rate. To address this, rates of stutter were contrasted against the LUS as well as the PAL on different flanking haplotypic backgrounds. This study shows that rates of stutter can vary substantially depending on the flanking haplotype, and while there are cases where the LUS is a better predictor of stutter than the PAL, examples to the contrary are apparent in commonly assayed forensic markers. Further, flanking variation that is 7 bp from the repeat region can impact rates of stutter. These findings suggest that non-proximal effects, such as DNA secondary structure, may be impacting the rates of stutter in common forensic short tandem repeat markers.
ISSN
:
2073-4425
Journal Name
:
Genes
Volume
:
8
Issue Number
:
11
Publishing Year
:
1438 AH
2017 AD
Article Type
:
Article
Added Date
:
Wednesday, June 26, 2019
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
August E. Woerner
Woerner, August E.
Investigator
Doctorate
August.Woerner@unthsc.edu
Jonathan L. King
King, Jonathan L.
Researcher
Doctorate
Bruce Budowle
Budowle, Bruce
Investigator
Doctorate
Bruce.Budowle@unthsc.edu
Files
File Name
Type
Description
44539.pdf
pdf
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